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lal chromogenic endotoxin quantification kit # 88282  (Thermo Fisher)


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    Thermo Fisher lal chromogenic endotoxin quantification kit # 88282
    Lal Chromogenic Endotoxin Quantification Kit # 88282, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chromogenic+endotoxin+quantification+kit/piercetm+chromogenic+endotoxin+quant+kit/pm40659660-543-7-12
    Average 90 stars, based on 1 article reviews
    lal chromogenic endotoxin quantification kit # 88282 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Recombinant:

    Article Title: Disruption of the blood-brain barrier contributes to neurobehavioral changes observed in rheumatic heart disease.
    Article Snippet: .. Recombinant GAS M5 protein was prepared as previously described22 and was determined to be free of LPS using Pierce Chromogenic Endotoxin Quantification Kit (ThermoFisher, USA) as per manufacturer's instructions. .. Immediately following ECG, rats were injected subcutaneously in the left hock with 500 μg of GAS rM5 emulsified 1:1 with complete Freund's adjuvant (CFA) (Sigma, USA).

    Article Title: Molecular basis for shifted receptor recognition by an encephalitic arbovirus.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-PCDH10 antibody Proteintech Cat#: 21859-1-AP; RRID: AB_2878929 Anti-VLDLR antibody GeneTex Cat#: GTX79552; RRID: AB_11171827 Anti-MXRA8 antibody MBL International Cat#: W040-3; RRID: AB_2801291 Rabbit IgG isotype Proteintech Cat#: 30000-0-AP; RRID: AB_2819035 Mouse IgG isotype BD Biosciences Cat#: BDB557351; RRID: AB_10050406 PE-conjugated donkey anti-rabbit F(ab’)2 fragment Jackson ImmunoResearch Cat#: 711-116-152; RRID: AB_2340599 PE-conjugated donkey anti-mouse F(ab’)2 fragment Jackson ImmunoResearch Cat#: 715-116-150; RRID: AB_2340798 APC-conjugated rat anti-DYKDDDDK (Flag) antibody BioLegend Cat#: 637307; RRID: AB_2561496 APC-conjugated rat isotype control antibody BioLegend Cat#: 402306; RRID: AB_3097097 Anti-Human IgG (Fc specific)–Peroxidase antibody Sigma Cat#: A0170; RRID: AB_257868 Anti-WEEV E2–E1 monoclonal antibody SKW11 Sutton et al.59 N/A Bacterial and virus strains Highlands J virus (strain B 230) ATCC Cat#: VR-622 Chemicals, peptides, and recombinant proteins DMEM Gibco Cat#: C11995500BT Expi293 Expression Medium Thermo Fisher Scientific Cat#: A1435101 Neurobasal medium Thermo Fisher Scientific Cat#: 21103 Glutamax Thermo Fisher Scientific Cat#: 35050 B-27 Thermo Fisher Scientific Cat#: 17504 Y-27632 Stemcell Technologies Cat#: 72302 Fetal bovine serum (FBS) ATLAS Biologicals Cat#: F-0500-D HEPES Thermo Fisher Scientific Cat#: 15630080 RPMI1640 Thermo Fisher Scientific Cat#: 11835055 Pen strep (penicillin-streptomycin) Gibco Cat#: 10378016 Opti-MEM Thermo Fisher Scientific Cat#: 31985088 Dulbecco’s Phosphate Buffered Saline (DPBS) Thermo Fisher Scientific Cat#: 14190-144 Pierce Chromogenic Endotoxin Quantification Kit Thermo Fisher Scientific Cat# A39552 MabSelectTM PrismA protein A affinity resin Cytiva Cat#: 17549801 Superdex 200 increase 10/300 GL column Cytiva Cat#: 28990944 Human PCDH10EC1-Fc Li et al. 15 N/A Human VLDLRLBD-Fc Clark et al. 34 N/A Human VLDLR LA(1–2)-Fc This study N/A Sparrow PCDH10EC1-Fc This study N/A Human MXRA8ect-Fc Clark et al. 34 N/A Human PCDH10EC1-twin-strep This study N/A Receptor associated protein (RAP) Clark et al.34 N/A Critical commercial assays ExpiFectamineTM 293 Transfection Kit Thermo Fisher Scientific Cat#: A14525 Lipofectamine 3000 Thermo Fisher Scientific Cat#: L3000150 e-Myco PCR detection kit Bulldog Bio Cat#: 25234 Deposited data WEEV CBA87 VLP density map This study EMD-47117 WEEV CBA87 VLP model This study PDB ID: 9DQX (Continued on next page) e1 Cell 188, 1–17.e1–e8, May 29, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER lentiGuide-Puro-garter snake PCDH10 Li et al.15 N/A lentiGuide-Puro-mouse PCDH10 Li et al.15 N/A lentiGuide-Puro horse PCDH10 Li et al.15 N/A WEEV CBA87 virus-like particle in pVRC plasmid Ko et al.32 N/A WEEV McMillan virus-like particle in pVRC plasmid Li et al.15 N/A WEEV Imperial 181 virus-like particle in pVRC plasmid This study N/A Western equine encephalitis virus (strain McMillan) molecular clone Gardner et al.60 N/A Software and algorithms RELION v3.1.4 Zivanov et al.61 N/A crYOLO v1.8.2 Wagner et al.62 N/A DeepEMhancer v20210511 Sanchez-Garcia et al.63 N/A MotionCor2 v1.6.4 Zheng et al.64 N/A CTFFIND-4.1 v4.1.14 Rohou and Grigorieff65 N/A UCSF ChimeraX v1.6.1 Pettersen et al.66 N/A PyMOL v3.0.2 https://www.pymol.org/pymol N/A Coot v0.9.8 Emsley et al.67 N/A Phenix v1.21rc1-5127 Adams et al.68 N/A AlphaFold2 Jumper et al.69 N/A GraphPad Prism v10.2.3 GraphPad N/A FlowJo v10.6.2 FlowJo, LLC N/A Incucyte S3 Software v2023B Sartorius N/A MEGA v11.0.10 MEGA Development Team N/A Other Quantifoil grids R 0.6/1 300 mesh, gold Electron Microscopy Sciences Cat#: Q350AR-06 Quantifoil grids R 2/2 300 mesh, gold Electron Microscopy Sciences Cat#: Q3100AR2 ll OPEN ACCESS Please cite this article in press as: Fan et al., Molecular basis for shifted receptor recognition by an encephalitic arbovirus, Cell (2025), https:// doi.org/10.1016/j.cell.2025.03.029 Article

    Article Title: Disruption of the blood–brain barrier contributes to neurobehavioral changes observed in rheumatic heart disease
    Article Snippet: .. Recombinant GAS M5 protein was prepared as previously described and was determined to be free of LPS using Pierce Chromogenic Endotoxin Quantification Kit (ThermoFisher, USA) as per manufacturer's instructions. .. Immediately following ECG, rats were injected subcutaneously in the left hock with 500 μg of GAS rM5 emulsified 1:1 with complete Freund's adjuvant (CFA) (Sigma, USA).

    other:

    Article Title: Ketogenic Diet‐Associated Worsening of Osteoarthritis Histologic Secerity, Increased Pain Sensitivity and Gut Microbiome Dysbiosis in Mice
    Article Snippet: A Pierce chromogenic endotoxin quantification kit was used to quantify LPS (Thermo Fisher) in LPS‐free plasticware.

    Control:

    Article Title: Molecular basis for shifted receptor recognition by an encephalitic arbovirus.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-PCDH10 antibody Proteintech Cat#: 21859-1-AP; RRID: AB_2878929 Anti-VLDLR antibody GeneTex Cat#: GTX79552; RRID: AB_11171827 Anti-MXRA8 antibody MBL International Cat#: W040-3; RRID: AB_2801291 Rabbit IgG isotype Proteintech Cat#: 30000-0-AP; RRID: AB_2819035 Mouse IgG isotype BD Biosciences Cat#: BDB557351; RRID: AB_10050406 PE-conjugated donkey anti-rabbit F(ab’)2 fragment Jackson ImmunoResearch Cat#: 711-116-152; RRID: AB_2340599 PE-conjugated donkey anti-mouse F(ab’)2 fragment Jackson ImmunoResearch Cat#: 715-116-150; RRID: AB_2340798 APC-conjugated rat anti-DYKDDDDK (Flag) antibody BioLegend Cat#: 637307; RRID: AB_2561496 APC-conjugated rat isotype control antibody BioLegend Cat#: 402306; RRID: AB_3097097 Anti-Human IgG (Fc specific)–Peroxidase antibody Sigma Cat#: A0170; RRID: AB_257868 Anti-WEEV E2–E1 monoclonal antibody SKW11 Sutton et al.59 N/A Bacterial and virus strains Highlands J virus (strain B 230) ATCC Cat#: VR-622 Chemicals, peptides, and recombinant proteins DMEM Gibco Cat#: C11995500BT Expi293 Expression Medium Thermo Fisher Scientific Cat#: A1435101 Neurobasal medium Thermo Fisher Scientific Cat#: 21103 Glutamax Thermo Fisher Scientific Cat#: 35050 B-27 Thermo Fisher Scientific Cat#: 17504 Y-27632 Stemcell Technologies Cat#: 72302 Fetal bovine serum (FBS) ATLAS Biologicals Cat#: F-0500-D HEPES Thermo Fisher Scientific Cat#: 15630080 RPMI1640 Thermo Fisher Scientific Cat#: 11835055 Pen strep (penicillin-streptomycin) Gibco Cat#: 10378016 Opti-MEM Thermo Fisher Scientific Cat#: 31985088 Dulbecco’s Phosphate Buffered Saline (DPBS) Thermo Fisher Scientific Cat#: 14190-144 Pierce Chromogenic Endotoxin Quantification Kit Thermo Fisher Scientific Cat# A39552 MabSelectTM PrismA protein A affinity resin Cytiva Cat#: 17549801 Superdex 200 increase 10/300 GL column Cytiva Cat#: 28990944 Human PCDH10EC1-Fc Li et al. 15 N/A Human VLDLRLBD-Fc Clark et al. 34 N/A Human VLDLR LA(1–2)-Fc This study N/A Sparrow PCDH10EC1-Fc This study N/A Human MXRA8ect-Fc Clark et al. 34 N/A Human PCDH10EC1-twin-strep This study N/A Receptor associated protein (RAP) Clark et al.34 N/A Critical commercial assays ExpiFectamineTM 293 Transfection Kit Thermo Fisher Scientific Cat#: A14525 Lipofectamine 3000 Thermo Fisher Scientific Cat#: L3000150 e-Myco PCR detection kit Bulldog Bio Cat#: 25234 Deposited data WEEV CBA87 VLP density map This study EMD-47117 WEEV CBA87 VLP model This study PDB ID: 9DQX (Continued on next page) e1 Cell 188, 1–17.e1–e8, May 29, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER lentiGuide-Puro-garter snake PCDH10 Li et al.15 N/A lentiGuide-Puro-mouse PCDH10 Li et al.15 N/A lentiGuide-Puro horse PCDH10 Li et al.15 N/A WEEV CBA87 virus-like particle in pVRC plasmid Ko et al.32 N/A WEEV McMillan virus-like particle in pVRC plasmid Li et al.15 N/A WEEV Imperial 181 virus-like particle in pVRC plasmid This study N/A Western equine encephalitis virus (strain McMillan) molecular clone Gardner et al.60 N/A Software and algorithms RELION v3.1.4 Zivanov et al.61 N/A crYOLO v1.8.2 Wagner et al.62 N/A DeepEMhancer v20210511 Sanchez-Garcia et al.63 N/A MotionCor2 v1.6.4 Zheng et al.64 N/A CTFFIND-4.1 v4.1.14 Rohou and Grigorieff65 N/A UCSF ChimeraX v1.6.1 Pettersen et al.66 N/A PyMOL v3.0.2 https://www.pymol.org/pymol N/A Coot v0.9.8 Emsley et al.67 N/A Phenix v1.21rc1-5127 Adams et al.68 N/A AlphaFold2 Jumper et al.69 N/A GraphPad Prism v10.2.3 GraphPad N/A FlowJo v10.6.2 FlowJo, LLC N/A Incucyte S3 Software v2023B Sartorius N/A MEGA v11.0.10 MEGA Development Team N/A Other Quantifoil grids R 0.6/1 300 mesh, gold Electron Microscopy Sciences Cat#: Q350AR-06 Quantifoil grids R 2/2 300 mesh, gold Electron Microscopy Sciences Cat#: Q3100AR2 ll OPEN ACCESS Please cite this article in press as: Fan et al., Molecular basis for shifted receptor recognition by an encephalitic arbovirus, Cell (2025), https:// doi.org/10.1016/j.cell.2025.03.029 Article

    Virus:

    Article Title: Molecular basis for shifted receptor recognition by an encephalitic arbovirus.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-PCDH10 antibody Proteintech Cat#: 21859-1-AP; RRID: AB_2878929 Anti-VLDLR antibody GeneTex Cat#: GTX79552; RRID: AB_11171827 Anti-MXRA8 antibody MBL International Cat#: W040-3; RRID: AB_2801291 Rabbit IgG isotype Proteintech Cat#: 30000-0-AP; RRID: AB_2819035 Mouse IgG isotype BD Biosciences Cat#: BDB557351; RRID: AB_10050406 PE-conjugated donkey anti-rabbit F(ab’)2 fragment Jackson ImmunoResearch Cat#: 711-116-152; RRID: AB_2340599 PE-conjugated donkey anti-mouse F(ab’)2 fragment Jackson ImmunoResearch Cat#: 715-116-150; RRID: AB_2340798 APC-conjugated rat anti-DYKDDDDK (Flag) antibody BioLegend Cat#: 637307; RRID: AB_2561496 APC-conjugated rat isotype control antibody BioLegend Cat#: 402306; RRID: AB_3097097 Anti-Human IgG (Fc specific)–Peroxidase antibody Sigma Cat#: A0170; RRID: AB_257868 Anti-WEEV E2–E1 monoclonal antibody SKW11 Sutton et al.59 N/A Bacterial and virus strains Highlands J virus (strain B 230) ATCC Cat#: VR-622 Chemicals, peptides, and recombinant proteins DMEM Gibco Cat#: C11995500BT Expi293 Expression Medium Thermo Fisher Scientific Cat#: A1435101 Neurobasal medium Thermo Fisher Scientific Cat#: 21103 Glutamax Thermo Fisher Scientific Cat#: 35050 B-27 Thermo Fisher Scientific Cat#: 17504 Y-27632 Stemcell Technologies Cat#: 72302 Fetal bovine serum (FBS) ATLAS Biologicals Cat#: F-0500-D HEPES Thermo Fisher Scientific Cat#: 15630080 RPMI1640 Thermo Fisher Scientific Cat#: 11835055 Pen strep (penicillin-streptomycin) Gibco Cat#: 10378016 Opti-MEM Thermo Fisher Scientific Cat#: 31985088 Dulbecco’s Phosphate Buffered Saline (DPBS) Thermo Fisher Scientific Cat#: 14190-144 Pierce Chromogenic Endotoxin Quantification Kit Thermo Fisher Scientific Cat# A39552 MabSelectTM PrismA protein A affinity resin Cytiva Cat#: 17549801 Superdex 200 increase 10/300 GL column Cytiva Cat#: 28990944 Human PCDH10EC1-Fc Li et al. 15 N/A Human VLDLRLBD-Fc Clark et al. 34 N/A Human VLDLR LA(1–2)-Fc This study N/A Sparrow PCDH10EC1-Fc This study N/A Human MXRA8ect-Fc Clark et al. 34 N/A Human PCDH10EC1-twin-strep This study N/A Receptor associated protein (RAP) Clark et al.34 N/A Critical commercial assays ExpiFectamineTM 293 Transfection Kit Thermo Fisher Scientific Cat#: A14525 Lipofectamine 3000 Thermo Fisher Scientific Cat#: L3000150 e-Myco PCR detection kit Bulldog Bio Cat#: 25234 Deposited data WEEV CBA87 VLP density map This study EMD-47117 WEEV CBA87 VLP model This study PDB ID: 9DQX (Continued on next page) e1 Cell 188, 1–17.e1–e8, May 29, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER lentiGuide-Puro-garter snake PCDH10 Li et al.15 N/A lentiGuide-Puro-mouse PCDH10 Li et al.15 N/A lentiGuide-Puro horse PCDH10 Li et al.15 N/A WEEV CBA87 virus-like particle in pVRC plasmid Ko et al.32 N/A WEEV McMillan virus-like particle in pVRC plasmid Li et al.15 N/A WEEV Imperial 181 virus-like particle in pVRC plasmid This study N/A Western equine encephalitis virus (strain McMillan) molecular clone Gardner et al.60 N/A Software and algorithms RELION v3.1.4 Zivanov et al.61 N/A crYOLO v1.8.2 Wagner et al.62 N/A DeepEMhancer v20210511 Sanchez-Garcia et al.63 N/A MotionCor2 v1.6.4 Zheng et al.64 N/A CTFFIND-4.1 v4.1.14 Rohou and Grigorieff65 N/A UCSF ChimeraX v1.6.1 Pettersen et al.66 N/A PyMOL v3.0.2 https://www.pymol.org/pymol N/A Coot v0.9.8 Emsley et al.67 N/A Phenix v1.21rc1-5127 Adams et al.68 N/A AlphaFold2 Jumper et al.69 N/A GraphPad Prism v10.2.3 GraphPad N/A FlowJo v10.6.2 FlowJo, LLC N/A Incucyte S3 Software v2023B Sartorius N/A MEGA v11.0.10 MEGA Development Team N/A Other Quantifoil grids R 0.6/1 300 mesh, gold Electron Microscopy Sciences Cat#: Q350AR-06 Quantifoil grids R 2/2 300 mesh, gold Electron Microscopy Sciences Cat#: Q3100AR2 ll OPEN ACCESS Please cite this article in press as: Fan et al., Molecular basis for shifted receptor recognition by an encephalitic arbovirus, Cell (2025), https:// doi.org/10.1016/j.cell.2025.03.029 Article

    Expressing:

    Article Title: Molecular basis for shifted receptor recognition by an encephalitic arbovirus.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-PCDH10 antibody Proteintech Cat#: 21859-1-AP; RRID: AB_2878929 Anti-VLDLR antibody GeneTex Cat#: GTX79552; RRID: AB_11171827 Anti-MXRA8 antibody MBL International Cat#: W040-3; RRID: AB_2801291 Rabbit IgG isotype Proteintech Cat#: 30000-0-AP; RRID: AB_2819035 Mouse IgG isotype BD Biosciences Cat#: BDB557351; RRID: AB_10050406 PE-conjugated donkey anti-rabbit F(ab’)2 fragment Jackson ImmunoResearch Cat#: 711-116-152; RRID: AB_2340599 PE-conjugated donkey anti-mouse F(ab’)2 fragment Jackson ImmunoResearch Cat#: 715-116-150; RRID: AB_2340798 APC-conjugated rat anti-DYKDDDDK (Flag) antibody BioLegend Cat#: 637307; RRID: AB_2561496 APC-conjugated rat isotype control antibody BioLegend Cat#: 402306; RRID: AB_3097097 Anti-Human IgG (Fc specific)–Peroxidase antibody Sigma Cat#: A0170; RRID: AB_257868 Anti-WEEV E2–E1 monoclonal antibody SKW11 Sutton et al.59 N/A Bacterial and virus strains Highlands J virus (strain B 230) ATCC Cat#: VR-622 Chemicals, peptides, and recombinant proteins DMEM Gibco Cat#: C11995500BT Expi293 Expression Medium Thermo Fisher Scientific Cat#: A1435101 Neurobasal medium Thermo Fisher Scientific Cat#: 21103 Glutamax Thermo Fisher Scientific Cat#: 35050 B-27 Thermo Fisher Scientific Cat#: 17504 Y-27632 Stemcell Technologies Cat#: 72302 Fetal bovine serum (FBS) ATLAS Biologicals Cat#: F-0500-D HEPES Thermo Fisher Scientific Cat#: 15630080 RPMI1640 Thermo Fisher Scientific Cat#: 11835055 Pen strep (penicillin-streptomycin) Gibco Cat#: 10378016 Opti-MEM Thermo Fisher Scientific Cat#: 31985088 Dulbecco’s Phosphate Buffered Saline (DPBS) Thermo Fisher Scientific Cat#: 14190-144 Pierce Chromogenic Endotoxin Quantification Kit Thermo Fisher Scientific Cat# A39552 MabSelectTM PrismA protein A affinity resin Cytiva Cat#: 17549801 Superdex 200 increase 10/300 GL column Cytiva Cat#: 28990944 Human PCDH10EC1-Fc Li et al. 15 N/A Human VLDLRLBD-Fc Clark et al. 34 N/A Human VLDLR LA(1–2)-Fc This study N/A Sparrow PCDH10EC1-Fc This study N/A Human MXRA8ect-Fc Clark et al. 34 N/A Human PCDH10EC1-twin-strep This study N/A Receptor associated protein (RAP) Clark et al.34 N/A Critical commercial assays ExpiFectamineTM 293 Transfection Kit Thermo Fisher Scientific Cat#: A14525 Lipofectamine 3000 Thermo Fisher Scientific Cat#: L3000150 e-Myco PCR detection kit Bulldog Bio Cat#: 25234 Deposited data WEEV CBA87 VLP density map This study EMD-47117 WEEV CBA87 VLP model This study PDB ID: 9DQX (Continued on next page) e1 Cell 188, 1–17.e1–e8, May 29, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER lentiGuide-Puro-garter snake PCDH10 Li et al.15 N/A lentiGuide-Puro-mouse PCDH10 Li et al.15 N/A lentiGuide-Puro horse PCDH10 Li et al.15 N/A WEEV CBA87 virus-like particle in pVRC plasmid Ko et al.32 N/A WEEV McMillan virus-like particle in pVRC plasmid Li et al.15 N/A WEEV Imperial 181 virus-like particle in pVRC plasmid This study N/A Western equine encephalitis virus (strain McMillan) molecular clone Gardner et al.60 N/A Software and algorithms RELION v3.1.4 Zivanov et al.61 N/A crYOLO v1.8.2 Wagner et al.62 N/A DeepEMhancer v20210511 Sanchez-Garcia et al.63 N/A MotionCor2 v1.6.4 Zheng et al.64 N/A CTFFIND-4.1 v4.1.14 Rohou and Grigorieff65 N/A UCSF ChimeraX v1.6.1 Pettersen et al.66 N/A PyMOL v3.0.2 https://www.pymol.org/pymol N/A Coot v0.9.8 Emsley et al.67 N/A Phenix v1.21rc1-5127 Adams et al.68 N/A AlphaFold2 Jumper et al.69 N/A GraphPad Prism v10.2.3 GraphPad N/A FlowJo v10.6.2 FlowJo, LLC N/A Incucyte S3 Software v2023B Sartorius N/A MEGA v11.0.10 MEGA Development Team N/A Other Quantifoil grids R 0.6/1 300 mesh, gold Electron Microscopy Sciences Cat#: Q350AR-06 Quantifoil grids R 2/2 300 mesh, gold Electron Microscopy Sciences Cat#: Q3100AR2 ll OPEN ACCESS Please cite this article in press as: Fan et al., Molecular basis for shifted receptor recognition by an encephalitic arbovirus, Cell (2025), https:// doi.org/10.1016/j.cell.2025.03.029 Article

    Saline:

    Article Title: Molecular basis for shifted receptor recognition by an encephalitic arbovirus.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-PCDH10 antibody Proteintech Cat#: 21859-1-AP; RRID: AB_2878929 Anti-VLDLR antibody GeneTex Cat#: GTX79552; RRID: AB_11171827 Anti-MXRA8 antibody MBL International Cat#: W040-3; RRID: AB_2801291 Rabbit IgG isotype Proteintech Cat#: 30000-0-AP; RRID: AB_2819035 Mouse IgG isotype BD Biosciences Cat#: BDB557351; RRID: AB_10050406 PE-conjugated donkey anti-rabbit F(ab’)2 fragment Jackson ImmunoResearch Cat#: 711-116-152; RRID: AB_2340599 PE-conjugated donkey anti-mouse F(ab’)2 fragment Jackson ImmunoResearch Cat#: 715-116-150; RRID: AB_2340798 APC-conjugated rat anti-DYKDDDDK (Flag) antibody BioLegend Cat#: 637307; RRID: AB_2561496 APC-conjugated rat isotype control antibody BioLegend Cat#: 402306; RRID: AB_3097097 Anti-Human IgG (Fc specific)–Peroxidase antibody Sigma Cat#: A0170; RRID: AB_257868 Anti-WEEV E2–E1 monoclonal antibody SKW11 Sutton et al.59 N/A Bacterial and virus strains Highlands J virus (strain B 230) ATCC Cat#: VR-622 Chemicals, peptides, and recombinant proteins DMEM Gibco Cat#: C11995500BT Expi293 Expression Medium Thermo Fisher Scientific Cat#: A1435101 Neurobasal medium Thermo Fisher Scientific Cat#: 21103 Glutamax Thermo Fisher Scientific Cat#: 35050 B-27 Thermo Fisher Scientific Cat#: 17504 Y-27632 Stemcell Technologies Cat#: 72302 Fetal bovine serum (FBS) ATLAS Biologicals Cat#: F-0500-D HEPES Thermo Fisher Scientific Cat#: 15630080 RPMI1640 Thermo Fisher Scientific Cat#: 11835055 Pen strep (penicillin-streptomycin) Gibco Cat#: 10378016 Opti-MEM Thermo Fisher Scientific Cat#: 31985088 Dulbecco’s Phosphate Buffered Saline (DPBS) Thermo Fisher Scientific Cat#: 14190-144 Pierce Chromogenic Endotoxin Quantification Kit Thermo Fisher Scientific Cat# A39552 MabSelectTM PrismA protein A affinity resin Cytiva Cat#: 17549801 Superdex 200 increase 10/300 GL column Cytiva Cat#: 28990944 Human PCDH10EC1-Fc Li et al. 15 N/A Human VLDLRLBD-Fc Clark et al. 34 N/A Human VLDLR LA(1–2)-Fc This study N/A Sparrow PCDH10EC1-Fc This study N/A Human MXRA8ect-Fc Clark et al. 34 N/A Human PCDH10EC1-twin-strep This study N/A Receptor associated protein (RAP) Clark et al.34 N/A Critical commercial assays ExpiFectamineTM 293 Transfection Kit Thermo Fisher Scientific Cat#: A14525 Lipofectamine 3000 Thermo Fisher Scientific Cat#: L3000150 e-Myco PCR detection kit Bulldog Bio Cat#: 25234 Deposited data WEEV CBA87 VLP density map This study EMD-47117 WEEV CBA87 VLP model This study PDB ID: 9DQX (Continued on next page) e1 Cell 188, 1–17.e1–e8, May 29, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER lentiGuide-Puro-garter snake PCDH10 Li et al.15 N/A lentiGuide-Puro-mouse PCDH10 Li et al.15 N/A lentiGuide-Puro horse PCDH10 Li et al.15 N/A WEEV CBA87 virus-like particle in pVRC plasmid Ko et al.32 N/A WEEV McMillan virus-like particle in pVRC plasmid Li et al.15 N/A WEEV Imperial 181 virus-like particle in pVRC plasmid This study N/A Western equine encephalitis virus (strain McMillan) molecular clone Gardner et al.60 N/A Software and algorithms RELION v3.1.4 Zivanov et al.61 N/A crYOLO v1.8.2 Wagner et al.62 N/A DeepEMhancer v20210511 Sanchez-Garcia et al.63 N/A MotionCor2 v1.6.4 Zheng et al.64 N/A CTFFIND-4.1 v4.1.14 Rohou and Grigorieff65 N/A UCSF ChimeraX v1.6.1 Pettersen et al.66 N/A PyMOL v3.0.2 https://www.pymol.org/pymol N/A Coot v0.9.8 Emsley et al.67 N/A Phenix v1.21rc1-5127 Adams et al.68 N/A AlphaFold2 Jumper et al.69 N/A GraphPad Prism v10.2.3 GraphPad N/A FlowJo v10.6.2 FlowJo, LLC N/A Incucyte S3 Software v2023B Sartorius N/A MEGA v11.0.10 MEGA Development Team N/A Other Quantifoil grids R 0.6/1 300 mesh, gold Electron Microscopy Sciences Cat#: Q350AR-06 Quantifoil grids R 2/2 300 mesh, gold Electron Microscopy Sciences Cat#: Q3100AR2 ll OPEN ACCESS Please cite this article in press as: Fan et al., Molecular basis for shifted receptor recognition by an encephalitic arbovirus, Cell (2025), https:// doi.org/10.1016/j.cell.2025.03.029 Article

    Transfection:

    Article Title: Molecular basis for shifted receptor recognition by an encephalitic arbovirus.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-PCDH10 antibody Proteintech Cat#: 21859-1-AP; RRID: AB_2878929 Anti-VLDLR antibody GeneTex Cat#: GTX79552; RRID: AB_11171827 Anti-MXRA8 antibody MBL International Cat#: W040-3; RRID: AB_2801291 Rabbit IgG isotype Proteintech Cat#: 30000-0-AP; RRID: AB_2819035 Mouse IgG isotype BD Biosciences Cat#: BDB557351; RRID: AB_10050406 PE-conjugated donkey anti-rabbit F(ab’)2 fragment Jackson ImmunoResearch Cat#: 711-116-152; RRID: AB_2340599 PE-conjugated donkey anti-mouse F(ab’)2 fragment Jackson ImmunoResearch Cat#: 715-116-150; RRID: AB_2340798 APC-conjugated rat anti-DYKDDDDK (Flag) antibody BioLegend Cat#: 637307; RRID: AB_2561496 APC-conjugated rat isotype control antibody BioLegend Cat#: 402306; RRID: AB_3097097 Anti-Human IgG (Fc specific)–Peroxidase antibody Sigma Cat#: A0170; RRID: AB_257868 Anti-WEEV E2–E1 monoclonal antibody SKW11 Sutton et al.59 N/A Bacterial and virus strains Highlands J virus (strain B 230) ATCC Cat#: VR-622 Chemicals, peptides, and recombinant proteins DMEM Gibco Cat#: C11995500BT Expi293 Expression Medium Thermo Fisher Scientific Cat#: A1435101 Neurobasal medium Thermo Fisher Scientific Cat#: 21103 Glutamax Thermo Fisher Scientific Cat#: 35050 B-27 Thermo Fisher Scientific Cat#: 17504 Y-27632 Stemcell Technologies Cat#: 72302 Fetal bovine serum (FBS) ATLAS Biologicals Cat#: F-0500-D HEPES Thermo Fisher Scientific Cat#: 15630080 RPMI1640 Thermo Fisher Scientific Cat#: 11835055 Pen strep (penicillin-streptomycin) Gibco Cat#: 10378016 Opti-MEM Thermo Fisher Scientific Cat#: 31985088 Dulbecco’s Phosphate Buffered Saline (DPBS) Thermo Fisher Scientific Cat#: 14190-144 Pierce Chromogenic Endotoxin Quantification Kit Thermo Fisher Scientific Cat# A39552 MabSelectTM PrismA protein A affinity resin Cytiva Cat#: 17549801 Superdex 200 increase 10/300 GL column Cytiva Cat#: 28990944 Human PCDH10EC1-Fc Li et al. 15 N/A Human VLDLRLBD-Fc Clark et al. 34 N/A Human VLDLR LA(1–2)-Fc This study N/A Sparrow PCDH10EC1-Fc This study N/A Human MXRA8ect-Fc Clark et al. 34 N/A Human PCDH10EC1-twin-strep This study N/A Receptor associated protein (RAP) Clark et al.34 N/A Critical commercial assays ExpiFectamineTM 293 Transfection Kit Thermo Fisher Scientific Cat#: A14525 Lipofectamine 3000 Thermo Fisher Scientific Cat#: L3000150 e-Myco PCR detection kit Bulldog Bio Cat#: 25234 Deposited data WEEV CBA87 VLP density map This study EMD-47117 WEEV CBA87 VLP model This study PDB ID: 9DQX (Continued on next page) e1 Cell 188, 1–17.e1–e8, May 29, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER lentiGuide-Puro-garter snake PCDH10 Li et al.15 N/A lentiGuide-Puro-mouse PCDH10 Li et al.15 N/A lentiGuide-Puro horse PCDH10 Li et al.15 N/A WEEV CBA87 virus-like particle in pVRC plasmid Ko et al.32 N/A WEEV McMillan virus-like particle in pVRC plasmid Li et al.15 N/A WEEV Imperial 181 virus-like particle in pVRC plasmid This study N/A Western equine encephalitis virus (strain McMillan) molecular clone Gardner et al.60 N/A Software and algorithms RELION v3.1.4 Zivanov et al.61 N/A crYOLO v1.8.2 Wagner et al.62 N/A DeepEMhancer v20210511 Sanchez-Garcia et al.63 N/A MotionCor2 v1.6.4 Zheng et al.64 N/A CTFFIND-4.1 v4.1.14 Rohou and Grigorieff65 N/A UCSF ChimeraX v1.6.1 Pettersen et al.66 N/A PyMOL v3.0.2 https://www.pymol.org/pymol N/A Coot v0.9.8 Emsley et al.67 N/A Phenix v1.21rc1-5127 Adams et al.68 N/A AlphaFold2 Jumper et al.69 N/A GraphPad Prism v10.2.3 GraphPad N/A FlowJo v10.6.2 FlowJo, LLC N/A Incucyte S3 Software v2023B Sartorius N/A MEGA v11.0.10 MEGA Development Team N/A Other Quantifoil grids R 0.6/1 300 mesh, gold Electron Microscopy Sciences Cat#: Q350AR-06 Quantifoil grids R 2/2 300 mesh, gold Electron Microscopy Sciences Cat#: Q3100AR2 ll OPEN ACCESS Please cite this article in press as: Fan et al., Molecular basis for shifted receptor recognition by an encephalitic arbovirus, Cell (2025), https:// doi.org/10.1016/j.cell.2025.03.029 Article

    Polymerase Chain Reaction:

    Article Title: Molecular basis for shifted receptor recognition by an encephalitic arbovirus.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-PCDH10 antibody Proteintech Cat#: 21859-1-AP; RRID: AB_2878929 Anti-VLDLR antibody GeneTex Cat#: GTX79552; RRID: AB_11171827 Anti-MXRA8 antibody MBL International Cat#: W040-3; RRID: AB_2801291 Rabbit IgG isotype Proteintech Cat#: 30000-0-AP; RRID: AB_2819035 Mouse IgG isotype BD Biosciences Cat#: BDB557351; RRID: AB_10050406 PE-conjugated donkey anti-rabbit F(ab’)2 fragment Jackson ImmunoResearch Cat#: 711-116-152; RRID: AB_2340599 PE-conjugated donkey anti-mouse F(ab’)2 fragment Jackson ImmunoResearch Cat#: 715-116-150; RRID: AB_2340798 APC-conjugated rat anti-DYKDDDDK (Flag) antibody BioLegend Cat#: 637307; RRID: AB_2561496 APC-conjugated rat isotype control antibody BioLegend Cat#: 402306; RRID: AB_3097097 Anti-Human IgG (Fc specific)–Peroxidase antibody Sigma Cat#: A0170; RRID: AB_257868 Anti-WEEV E2–E1 monoclonal antibody SKW11 Sutton et al.59 N/A Bacterial and virus strains Highlands J virus (strain B 230) ATCC Cat#: VR-622 Chemicals, peptides, and recombinant proteins DMEM Gibco Cat#: C11995500BT Expi293 Expression Medium Thermo Fisher Scientific Cat#: A1435101 Neurobasal medium Thermo Fisher Scientific Cat#: 21103 Glutamax Thermo Fisher Scientific Cat#: 35050 B-27 Thermo Fisher Scientific Cat#: 17504 Y-27632 Stemcell Technologies Cat#: 72302 Fetal bovine serum (FBS) ATLAS Biologicals Cat#: F-0500-D HEPES Thermo Fisher Scientific Cat#: 15630080 RPMI1640 Thermo Fisher Scientific Cat#: 11835055 Pen strep (penicillin-streptomycin) Gibco Cat#: 10378016 Opti-MEM Thermo Fisher Scientific Cat#: 31985088 Dulbecco’s Phosphate Buffered Saline (DPBS) Thermo Fisher Scientific Cat#: 14190-144 Pierce Chromogenic Endotoxin Quantification Kit Thermo Fisher Scientific Cat# A39552 MabSelectTM PrismA protein A affinity resin Cytiva Cat#: 17549801 Superdex 200 increase 10/300 GL column Cytiva Cat#: 28990944 Human PCDH10EC1-Fc Li et al. 15 N/A Human VLDLRLBD-Fc Clark et al. 34 N/A Human VLDLR LA(1–2)-Fc This study N/A Sparrow PCDH10EC1-Fc This study N/A Human MXRA8ect-Fc Clark et al. 34 N/A Human PCDH10EC1-twin-strep This study N/A Receptor associated protein (RAP) Clark et al.34 N/A Critical commercial assays ExpiFectamineTM 293 Transfection Kit Thermo Fisher Scientific Cat#: A14525 Lipofectamine 3000 Thermo Fisher Scientific Cat#: L3000150 e-Myco PCR detection kit Bulldog Bio Cat#: 25234 Deposited data WEEV CBA87 VLP density map This study EMD-47117 WEEV CBA87 VLP model This study PDB ID: 9DQX (Continued on next page) e1 Cell 188, 1–17.e1–e8, May 29, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER lentiGuide-Puro-garter snake PCDH10 Li et al.15 N/A lentiGuide-Puro-mouse PCDH10 Li et al.15 N/A lentiGuide-Puro horse PCDH10 Li et al.15 N/A WEEV CBA87 virus-like particle in pVRC plasmid Ko et al.32 N/A WEEV McMillan virus-like particle in pVRC plasmid Li et al.15 N/A WEEV Imperial 181 virus-like particle in pVRC plasmid This study N/A Western equine encephalitis virus (strain McMillan) molecular clone Gardner et al.60 N/A Software and algorithms RELION v3.1.4 Zivanov et al.61 N/A crYOLO v1.8.2 Wagner et al.62 N/A DeepEMhancer v20210511 Sanchez-Garcia et al.63 N/A MotionCor2 v1.6.4 Zheng et al.64 N/A CTFFIND-4.1 v4.1.14 Rohou and Grigorieff65 N/A UCSF ChimeraX v1.6.1 Pettersen et al.66 N/A PyMOL v3.0.2 https://www.pymol.org/pymol N/A Coot v0.9.8 Emsley et al.67 N/A Phenix v1.21rc1-5127 Adams et al.68 N/A AlphaFold2 Jumper et al.69 N/A GraphPad Prism v10.2.3 GraphPad N/A FlowJo v10.6.2 FlowJo, LLC N/A Incucyte S3 Software v2023B Sartorius N/A MEGA v11.0.10 MEGA Development Team N/A Other Quantifoil grids R 0.6/1 300 mesh, gold Electron Microscopy Sciences Cat#: Q350AR-06 Quantifoil grids R 2/2 300 mesh, gold Electron Microscopy Sciences Cat#: Q3100AR2 ll OPEN ACCESS Please cite this article in press as: Fan et al., Molecular basis for shifted receptor recognition by an encephalitic arbovirus, Cell (2025), https:// doi.org/10.1016/j.cell.2025.03.029 Article



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